reverse transcriptase m-mlv mutants superscript ii Search Results


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GenScript corporation rice codon-optimized m-mlv rt d200n/l603w/t330p/t306k/w313f mutant
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Operon Biotech mutant moloney murine leukemia virus reverse transcriptase gene m-mlv rt
Multiple site-directed mutagenesis of a <t>M-MLV</t> RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.
Mutant Moloney Murine Leukemia Virus Reverse Transcriptase Gene M Mlv Rt, supplied by Operon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher superscript™ ii mutant m-mlv rt
Multiple site-directed mutagenesis of a <t>M-MLV</t> RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.
Superscript™ Ii Mutant M Mlv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega m-mlv point mutant
Multiple site-directed mutagenesis of a <t>M-MLV</t> RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.
M Mlv Point Mutant, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega m-mlv reverse transcriptase rnase h minus mutant
Multiple site-directed mutagenesis of a <t>M-MLV</t> RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.
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Qiagen m mlv reverse transcriptase
Multiple site-directed mutagenesis of a <t>M-MLV</t> RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.
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Multiple site-directed mutagenesis of a M-MLV RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.

Journal: BMC Biotechnology

Article Title: Patch cloning method for multiple site-directed and saturation mutagenesis

doi: 10.1186/1472-6750-13-91

Figure Lengend Snippet: Multiple site-directed mutagenesis of a M-MLV RT gene. Schematic presentation of six- (a) or nine-point (b) simultaneous mutation in M-MLV RT gene is shown. DNA fragments amplified by PCR were assembled with the NdeI–XhoI-digested pET16b vector using MUPAC. Length in base pair is shown over each DNA fragment. Plasmids were extracted from each six- and nine-point site-directed mutagenesis experiment. After digestion with NdeI and XhoI, the digested plasmids were analyzed using 1.2% agarose gel electrophoresis (lanes 2–17, the six-site experiments; lanes 19–34, the nine-site experiment). Lane 1 and 18 contain 1000 bp DNA ladder marker. CFU in each experiment are listed in Additional file : Table S3. (c) Summary of 6- or 9-points mutation. Mutants were analyzed using electrophoresis and DNA sequencing.

Article Snippet: Mutant Moloney Murine Leukemia Virus reverse transcriptase gene (M-MLV RT, Additional file : Figure S9) [ , ] was purchased from Operon Biotechnologies (Alameda, CA) and was cloned into pET16b (Novagen, Darmstadt, Germany) between NdeI and XhoI sites to construct pET16b-MMLVRT.

Techniques: Mutagenesis, Amplification, Plasmid Preparation, Agarose Gel Electrophoresis, Marker, Electrophoresis, DNA Sequencing